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source directly to the specimen illuminating it to produce a sharp image. The condenser is mostly used in high-resolution microscopes and in the case of oil immersion microscopy the condenser may be immersed in particular oil to increase the image resolution. On the other hand, the iris diaphragm is a rotating disk under the stage with different sized holes and serves to regulate the amount of light coming from the condenser to ensure that only the required amount reaches the specimen to acquire the desired image contrast. For light microscopes with low-resolution power, the diaphragm is replaced with a single standard opening to allow a constant amount of light to the specimen. 4. The difference...

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