Protein Essay Examples

protein molecular mass ranges of between 1000 and 50000 as such 6-75 Sephadex is also applied. The molecules that are small in size can fit into the porous beads and can be said to be included. On the other hand, the more important molecules cannot fit into the dots and are excluded. The small molecules have access to the mobile phase and are eluted last. Equations The essential equations that form the basis of the experiment are: Vt=Vo+ViWhere Vt is the total volume of the liquid in the column. Vo is the volume of the column void while Vi is the volume inside the gel. The equation is possible because the buffer in the gel filtration is seen as the partitioning outside and inside the gels. To...

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