The Antibacterial and Antifungal Activities

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The antibacterial and antifungal activities were evaluated by standardized disc-agar diffusion method . The efficacy of the novel antimicrobial compounds was determined against different strains of gram-positive and gram-negative bacteria. These compounds were also evaluated against different fungal strains. The gram-positive organisms that were used for culture sensitivity include Staphylococcus aureus (RCMB 010027), Streptococcus pneumoniae (RCMB 010010) and Bacillis subtilis (RCMB 010067). On the other hand, the gram-negative organisms that were used for culture sensitivity include Pseudomonas aeruginosa (RCMB 010043), Klebsiella pneumoniae (RCMB 0010093) and Escherichia coli (RCMB 010052) and Sallmonella typhimurium (RCMB 010315. The fungal strains that were used include Aspergillus fumigatus (RCMB 02568) and Candida albicans (RCMB 05036). Different antibiotics were used as reference for evaluating the antimicrobial activity of novel compounds. Ampicillin was used as the reference antibiotic for assessing the antimicrobial activity of the novel compounds against gram-positive bacteria. Gentamicin was used as the reference antibiotic for assessing the antimicrobial activity of the novel compounds against gram-negative bacteria. On the other hand, Amphotericin B was used as the reference antibiotic for assessing the antimicrobial activity of the novel compounds against for fungal strains. Assay of Antimicrobial activity M. tuberculosis (RCMB 010126) was used as the experimental microbe for the present study. Pyrazinamide and Isoniazid were used as the reference antibiotic in comparison to the novel compounds. MABA (Microbial Alamar Blue Assay) was conducted to evaluate

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