The Research Problem(s)

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The Research Problem(s). The research problem entails ascertaining the genetic bases of alcohol use disorder (AUD). The Hypothesis of the Research The study hypothesizes that ancestral alcohol exposure influences the consumption of alcohol, as well as neurobiological sensitivity to alcohol. The Importance of the Research The research is important because it highlights the correlation between paternal alcoholism and its influence on children sired by alcoholics. Moreover, the study contributes to the literature on genetically acquired attributes related to alcohol consumption, how such attributes influence certain behaviors, as well as and how the outcomes vary according to gender. Methods and Procedures The research was conducted using real-time Quantitative PCR (RT-Qpcr) in which RNA was changed into cDNA, the process involved the calculation of duplicate genes and finding the mean of the resulting values. The method employed the two-bottle choice test in determining the effect of ethanol exposure on ethanol predilection and use. To test the research hypothesis, the researchers exposed male mice to continual vapor ethanol and to control conditions, after which they were mated with “ethanol-naïve” females. Their offspring were then tested for ethanol-induced behaviors, ethanol drinking, DNA methylation, and gene expression. Evaluation of Methods and Procedures The methods and procedures were significant in establishing whether alcoholic behaviors are heritable and whether the behaviors develop subsequently in the sired offsprings. The procedures were important because they helped ascertain the genetic bases of alcohol use disorder. Sample To conduct the experiment,

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