Antimicrobial investigation

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Antimicrobial investigation Standardized disc-agar was applied with the intention of conducting antimicrobial investigation, focusing on it as a tool for in vitro assessment. In this case, it was used to evaluate the activity of selected organisms by looking at synthesized compounds from selected microorganisms. The organisms comprised of fungi, gram negative, and gram positive bacteria to include Aspergillus fumigatus (RCMB 02568), Salmonella typhimurium (RCMB 010315), Mycobactrium tuberculosis (RCMB010120), Klebsiella pneumoniae (RCMB 0010093), Escherichia coli (RCMB 010052), Bacillis subtilis (RCMB 010067), Streptococcus pneumoniae (RCMB 010010), and Staphylococcus aureus (RCMB 010027). For the gram negative bacteria, antibiotic penicillin was applied as the standard reference while gentamicin was applied for gram positive bacteria. Amphoteric B was applied for all bacteria samples as the standard antifungal control. The synthesized compounds were then collected by dissolving in DMF at concentrations of 1 mg mL-1 and 5 mg mL-1 before commencing testing. Evaluation of the anticancer activity The synthesized compounds (dissolved in DMF) were assessed for cytotoxic activity against colon carcinoma (HCT-116) and breast carcinoma (MCF-7) cell lines as the experimental groups. The experiment used Baby Hamster Kidney (BHK) cells as the controls since they are a normal cell line. The evaluation for the experimental and control cells focused on the ratio for inhibition of cell growth (IC50) by applying the formula: (C-T / C) x 100 = IC50. Within the IC50 formula, the C implies mean absorbance for the untreated BHK cells as controls while the T implies the mean absorbance for

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